For the operational flight tests was planned to Vandenberg AFB in California. Since 1958 there has been work on the Operational Suitability Test Facility (OSTF). This system should provide the first experience with the handling of Titan missiles in their silos and launch it. The OSTF still did not meet the planned stationing of Silokonfiguration sites, but this was very similar. After filling the tank of the missile in the silo, a large hydraulic elevator lift the rocket out of its silo and then they will be fired. At a fueling test on 3 December 1960 drove the missile from the silo should be lowered and then again, it failed, the hydraulic lift and the rocket fell uncontrollably from fully fueled in the silo. In the ensuing explosion, the plant was completely destroyed. At a reconstruction has been omitted. On 3 May 1961 was the first launch of a Titan I silo launch from the Vandenberg Test Facility (SLTF). A specially modified for this launch rocket was launched as the different operational later in the Titan I silo JET AFS-1000B. This test formed the basis for the later deployment of the concept and was the only Titan II silo in the launch of a Titan I, on 23 September 1961 there was the first launch of a Titan I from the now completed Titan I Training Facility (TF-1, Launch Complex 395-A1, A2, A3) at Vandenberg, which corresponded to the operational silos. On 29 January 1962 ended the test flight program of the Titan I on the Cape Canaveral AFS, Florida. After that only operational tests from Vandenberg AFB have been carried out, which ended with the end of the Titan I program 1965th In total there were 47 Titan I development flights, of which 32 successfully, ten partially successful and five failures.
Titan I missiles were deployed in missile complexes, of which three Strategic Missile Squadron, one (SMS) formed. The USAF demanded first SMS after eleven, in the meantime even deletion was up in a text message or the complete deletion of the program because of its high cost this week. Finally, 54 missiles were deployed in six SMS. The locations for Titan I missiles were the Lowry AFB (2 SMS), Mountain Home AFB, Beale AFB, Larson AFB and Ellsworth AFB. The silo at Vandenberg AFB have also been put on alert status, but they served, except for a brief period during the Cuban missile crisis only for training purposes.