ELISA Kits and what they are used for
Enzyme-linked immunosorbent assay (ELISA) is what is referred to as an affinity-based method, normally conducted using multi-well plates, which serve to determine if an antigen, frequently an antibody or a protein, is present within the sample.
Generally, the method for ELISA will begin with an antigen being immobilized in a multi-well plate. Next, the whole sample may be allowed to settle, at which point it adheres to the plate. Otherwise, an antibody which is specific to the antigen may be bound to the plate. This then captures the antigen and the antigen becomes immobilized.
ELISA kits are available with an extensive array of portfolio ranges which come as neurobiology-related as well as phosphor-specific kits and additionally cytokine/ chemokine kits.
ELISA kits aid in the provision of accurate and consistent results. The target proteins in turn are researched and the kits have been calibrated in order to give physiologically relevant sensitivity.
Moreover, the kits are validated by way of common sample types, which includes plasma, serum, and cell culture supernatant. Validation occurs through the use of cell lysates. Cell lysates detect phosphorylation or signaling proteins.
All ELISA kits have to meet rigorous quality control specifications through strict regulatory control. They are manufactured with stringent quality controls in an ISO facility which helps to ensure very high quality and reproducibility.
Benefits of ELISA Kits
Many ELISA kits on the market today come with an array of benefits:
- Broad ranging menus of over 800 targets
- Highly optimized to give accurate, consistent, and sensitive performance levels
- Comprehensive instructions which help to complete the protocol in a timely manner, though timing does vary by kit
- Kits are validated for a variety of typical sample types – supernatant, plasma, lysates, serum, for example
What ELISA Kits Normally Include
ELISA kits routinely come with:
- An antibody-coated 96-well plate
- A primary detection antibody which is typically biotinylated
- A secondary detection reagent which is typically streptavidin-HRP
- Wash buffers
- Diluent buffers
- Stop solutions and substrate
- Plate covers
Most kits on the market are effective for a variety of sample types, for example, plasma, serum, as well as cell culture supernatant, or tissue homogenate, cell lysate, cerebrospinal fluid (CSF), and urine. These are appropriate to the target.
Kits are generally available for various uses – human, monkey, mouse, rat, porcine (swine), and bovine samples, while a number of kits are designed to be used with multiple species.
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